Endophytes of indigenous grapevines as a novel source of biopesticides in viticulture

Summary

Objective

1. Microbiome Characterisation: Use Next-Generation Sequencing to profile the taxonomic and functional diversity of endophytes in indigenous and commercial Australian grapevines.
2. Bioactivity Investigation: Conduct bioassays (in vitro and in planta) and genome sequencing to identify endophytes with beneficial biosynthetic potential.
3. Metabolic Screening: Analyse the biochemical diversity of endophytes with antimicrobial qualities to assess their agrochemical applications.

Background

As the wine industry seeks sustainable solutions to reduce chemical inputs, endophytes—symbiotic microorganisms living within plant tissues—are emerging as a promising alternative to traditional pesticides. These naturally occurring microbes enhance plant resilience by boosting defence mechanisms, improving nutrient uptake, and helping vines withstand environmental stresses such as drought and salinity. Unlike chemical pesticides, which can pose risks to human health, the environment, and the winemaking process, endophytes offer a more targeted, long-lasting approach to vineyard protection.

Key benefits of endophytes include:

• Sustainable Pest Management – They provide long-term resistance against pathogens and pests.
• Eco-Friendly Approach – They minimise harm to beneficial organisms and non-target species.
• Reduced Resistance Risks – They lower the chances of pathogens developing resistance compared to conventional pesticides.

While some biocontrol agents, such as Trichoderma sp. and Bacillus sp., are already used in viticulture, there has been little exploration of endophytes native to Australian grapevines. Studies on wild grape species from Asia and North America have identified endophytes that combat key Vitis vinifera pathogens, such as Botrytis cinerea and Ralstonia syzygii. This suggests that Australia’s indigenous grape microbiome could hold untapped potential for developing natural, vineyard-specific biocontrol solutions.

By investigating the microbial diversity within native Australian grapevines, this research aims to uncover novel endophytes that can enhance vineyard health, reduce chemical reliance, and future-proof the wine industry against emerging threats.

Sector benefits

This project aims to explore the internal microbiome of both Australian indigenous and commercial grapevines to identify beneficial endophytes with potential applications in viticulture. Through advanced sequencing, bioassays, and metabolic screening, the research will assess the antimicrobial properties of these microorganisms and their potential as biological alternatives to chemical pesticides.